潘伟伟,李甜女,冯靖

• 1:南京医科大学附属淮安第一医院三分院口腔科

摘要(Abstract)：

目的 探究甘草甜素（glycyrrhizin,GL）通过调节核因子-κB(nuclear factor-κB, NF-κB)/核苷酸结合寡聚结构域样受体蛋白3(nucleotide-binding oligomerization domain-like receptor protein 3, NLRP3)/白细胞介素（interleukin, IL）-1β信号通路对慢性牙周炎（chronic periodontitis, CP）大鼠牙周组织损伤的影响。方法 取部分大鼠经牙龈卟啉单胞菌感染建立模型，将建模成功的60只大鼠随机分为模型组、低剂量GL(low-dose GL, L-GL)组、中剂量GL(medium-dose GL, M-GL)组、高剂量GL(highdose GL, H-GL)组（低、中、高剂量组分别静脉注射2、4、10 mg·kg~(-1) GL）、通路激活剂组（静脉注射0.5 mg·kg~(-1) NF-κB/NLRP3/IL-1β通路激活剂CHPG），每组12只;另随机选取12只大鼠作为对照组;其中对照组和模型组静脉注射等量生理盐水，每日1次，持续20 d。采用探针测定牙周探诊深度（probing depth, PD）;观察牙齿活动度（tooth mobility, TM）;评估出血指数（bleeding index, BI）;通过碱性品红染色法测定菌斑指数（plaque index, PLI）;通过酶联免疫吸附试验（enzyme linked immunosorbent assay, ELISA）检测血清中IL-1β、IL-6、干扰素-γ(interferon-γ, IFN-γ)水平;显微计算机断层扫描（micro computed tomography, Micro-CT）分析牙槽骨吸收情况;采用苏木精-伊红（hematoxylin-eosin, HE）染色和抗酒石酸酸性磷酸酶（tartrate resistant acid phosphatase, TRAP）染色观察牙周组织的病理学变化及破骨细胞数量;采用免疫组织化学技术（immunohistochemistry, IHC）和蛋白印迹法（Western blotting）检测牙周组织中NF-κB、NLRP3、IL-1β蛋白的阳性率及表达水平。结果 模型组大鼠出现上皮破裂及大量炎症细胞浸润，提示建模成功;与对照组比较，模型组的IL-1β、IFN-γ、IL-6、PD、TM、BI、PLI、牙骨质-釉质交界（cemento-enamel junction, CEJ）、CEJ到牙槽骨顶（alveolar ridge, ABC）的距离（CEJ-ABC）值、破骨细胞数量，以及NF-κB、NLRP3、IL-1β蛋白的阳性率和表达水平均显著升高（P<0.05）;与模型组比较，L-GL组、M-GL组、HGL组的牙周组织损伤得到改善，上述指标均显著降低（P<0.05）;通路激活剂则可逆转GL的处理效果。结论 GL可能通过抑制NF-κB/NLRP3/IL-1β通路改善CP大鼠的牙周组织损伤，且该作用呈浓度依赖性。

关键词(KeyWords)： 甘草甜素;慢性牙周炎;核因子-κB/核苷酸结合寡聚结构域样受体蛋白3/白细胞介素-1β信号通路;牙周组织损伤;炎症

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 潘伟伟,李甜女,冯靖

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